PIPES Buffer 0.5M,pH7.5,0.5M,pH8.0,1.0M,pH6.0,1.0M,pH6.5,1.0M,pH6.8,1.0M,pH7.0,1.0M,pH7.4,1.0M,pH7.5,1.0M,pH8.0,0.5M, pH5.5,0.5M, pH6.0,0.5M, pH6.5,0.5M, pH6.8,0.5M, pH7.0,0.5M, pH7.4,5mM,pH=8